Žučna-kiselina 7alfa-dehidroksilaza
(Preusmjereno sa stranice Žučno-kiselinska 7alfa-dehidroksilaza)
Žučna-kiselina 7alfa-dehidroksilaza (EC 1.17.99.5, holatna 7alfa-dehidroksilaza, 7alfa-dehidroksilaza, žučno kiselinska 7-dehidroksilaza) je enzim sa sistematskim imenom dezoksiholat:NAD+ oksidoreduktaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Žučna-kiselina 7alfa-dehidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.17.99.5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) dezoksiholat + NAD+ + H2O holat + NADH + H+
- (2) lithoholat + NAD+ +H2O henodezoksiholat + NADH + H+
Ovaj enzim je visoko specifičan za žučno-kiselinske supstrate. Za njegovo dejstvo je neophodna slobodna C-24 karboksilna grupa i nezaklonjena 7alfa-hidroksi grupa na B-prstenu steroid jezgra.
Reference
uredi- ↑ White, B.A., Cacciapuoti, A.F., Fricke, R.J., Whitehead, T.R., Mosbach, E.H. and Hylemon, P.B. (1981). „Cofactor requirements for 7α-dehydroxylation of cholic and chenodeoxycholic acid in cell extracts of the intestinal anaerobic bacterium, Eubacterium species V.P.I. 12708”. J. Lipid Res. 22: 891-898. PMID 7276750.
- ↑ White, B.A., Paone, D.A., Cacciapuoti, A.F., Fricke, R.J., Mosbach, E.H. and Hylemon, P.B. (1983). „Regulation of bile acid 7-dehydroxylase activity by NAD+ and NADH in cell extracts of Eubacterium species V.P.I. 12708”. J. Lipid Res. 24: 20-27. PMID 6833878.
- ↑ Coleman, J.P., White, W.B. and Hylemon, P.B. (1987). „Molecular cloning of bile acid 7-dehydroxylase from Eubacterium sp. strain VPI 12708”. J. Bacteriol. 169: 1516-1521. PMID 3549693.
- ↑ Russell, D.W. (2003). „The enzymes, regulation, and genetics of bile acid synthesis”. Annu. Rev. Biochem. 72: 137-174. PMID 12543708.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.