Tropinon reduktaza I
Tropinon reduktaza I (EC 1.1.1.293, tropinonska reduktaza, TR-I) je enzim sa sistematskim imenom tropin:NADP+ oksidoreduktaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Tropinon reduktaza I | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.293 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- tropin + NADP+ tropinon + NADPH + H+
Ovaj enzim zajedno sa EC 1.1.1.236, tropinonskom reduktazom II, predstavlja tačku grananja u metabolizmu tropanskih alkaloida. Tropin (produkt enzima EC 1.1.1.293) se inkorporiraj u hiosciamin i skopolamin, dok je pseudotropin (produkt enzima EC 1.1.1.236) prvi specifični metabolit na putu do kalistegina. Oba enzima su uvek zajedno prisutna kod vrsta koje formiraju tropanske alkaloide. Oni imaju zajednički supstrat, tropinon, i strogo su stereospecifični.
Reference uredi
- ↑ Couladis, M.M, Friesen, J.B., Landgrebe, M.E. and Leete, E. (1991). „Enzymes catalysing the reduction of tropinone to tropine and ψ-tropine isolated from the roots of Datura innoxia”. Pytochemistry 30: 801-805.
- ↑ Nakajima, K., Hashimoto, T. and Yamada, Y. (1993). „Two tropinone reductases with different stereospecificities are short-chain dehydrogenases evolved from a common ancestor”. Proc. Natl. Acad. Sci. USA 90: 9591-9595. PMID 8415746.
- ↑ Dräger, B. (2006). „Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism”. Phytochemistry 67: 327-337. PMID 16426652.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.