Protein N-terminal metiltransferaza
Protein N-terminal metiltransferaza (EC 2.1.1.244, NMT1 (gen), METTL11A (gen)) je enzim sa sistematskim imenom S-adenozil-L-metionin:N-terminal-(A,P,S)PK-(protein) metiltransferaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Protein N-terminal metiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.244 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) 3 S-adenozil-L-metionin + N-terminal-(A,S)PK-[protein] 3 S-adenozil-L-homocistein + N-terminal-N,N,N-trimetil-N-(A,S)PK-[protein] (sveukupna reakcija)
- (1a) S-adenozil-L-metionin + N-terminal-(A,S)PK-[protein] S-adenozil-L-homocistein + N-terminal-N-metil-N-(A,S)PK-[protein]
- (1b) S-adenozil-L-metionin + N-terminal-N-metil-N-(A,S)PK-[protein] S-adenozil-L-homocistein + N-terminal-N,N-dimetil-N-(A,S)PK-[protein]
- (1c) S-adenozil-L-metionin + N-terminaL-N,N-dimetil-N-(A,S)PK-serin-[protein] S-adenozil-L-homocistein + N-terminal-N,N,N-trimetil-N-(A,S)PK-[protein]
- (2) 2 S-adenozil-L-metionin + N-terminal-PPK-[protein] 2 S-adenozil-L-homocistein + N-terminal-N,N-dimetil-N-PPK-[protein] (sveukupna reakcija)
- (2a) S-adenozil-L-metionin + N-terminal-PPK-[protein] S-adenozil-L-homocistein + N-terminal-N-metil-N-PPK-[protein]
- (2b) S-adenozil-L-metionin + N-terminal-N-metil-N-PPK-[protein] S-adenozil-L-homocistein + N-terminal-N,N-dimetil-N-PPK-[protein]
Ovaj enzim metiliše N-terminus ciljnih proteina koji sadrže N-terminalni motiv [Ala/Pro/Ser]-Pro-Lys nakon odsecanja inicijatora L-metionina. Kad je terminalna aminokiselina L-prolin, ovaj enzim katalizuje dve uzastopne metilacije alfa-amino grupa.
Reference uredi
- ↑ Webb, K.J., Lipson, R.S., Al-Hadid, Q., Whitelegge, J.P. and Clarke, S.G. (2010). „Identification of protein N-terminal methyltransferases in yeast and humans”. Biochemistry 49: 5225-5235. PMID 20481588.
- ↑ Tooley, C.E., Petkowski, J.J., Muratore-Schroeder, T.L., Balsbaugh, J.L., Shabanowitz, J., Sabat, M., Minor, W., Hunt, D.F. and Macara, I.G. (2010). „NRMT is an α-N-methyltransferase that methylates RCC1 and retinoblastoma protein”. Nature 466: 1125-1128. PMID 20668449.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.