Glicin/sarkozin N-metiltransferaza
Glicin/sarkozin N-metiltransferaza (EC 2.1.1.156, ApGSMT, glicin-sarkozinska metiltransferaza, GSMT, GMT, glicin sarkozinska N-metiltransferaza, S-adenozil-L-metionin:sarkozin N-metiltransferaza) je enzim sa sistematskim imenom S-adenozil-L-metionin:glicin(or sarkozin) N-metiltransferaza (sarkozin(or formira N,N-dimetilglicin)).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Glicin/sarkozin N-metiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.156 | ||||||||
| CAS broj | 294210-82-5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 2 S-adenozil-L-metionin + glicin 2 S-adenozil-L-homocistein + N,N-dimetilglicin (sveukupna reakcija)
- (1a) S-adenozil-L-metionin + glicin S-adenozil-L-homocistein + sarkozin
- (1b) S-adenozil-L-metionin + sarkozin S-adenozil-L-homocistein + N,N-dimetilglicin
Cijanobakterija Aphanocthece halophytica sintetiše betain iz glicina putem metilacije koja se odvija u tri koraka. Ovaj enzim posreduje prvi korak te sekvence.
Reference uredi
- ↑ Nyyssölä, A., Kerovuo, J., Kaukinen, P., von Weymarn, N. and Reinikainen, T. (2000). „Extreme halophiles synthesize betaine from glycine by methylation”. J. Biol. Chem. 275: 22196-22201. PMID 10896953.
- ↑ Nyyssölä, A., Reinikainen, T. and Leisola, M. (2001). „Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase.”. Appl. Environ. Microbiol. 67: 2044-2050. PMID 11319079.
- ↑ Waditee, R., Tanaka, Y., Aoki, K., Hibino, T., Jikuya, H., Takano, J., Takabe, T. and Takabe, T. (2003). „Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica”. J. Biol. Chem. 278: 4932-4942. PMID 12466265.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.