Nukleaza
Nukleaza je enzim koji razlaže fosfodiestarske veze između nukleotidnih podjedinica nukleinskih kiselina. U starijoj literaturi se koriste termini kao što su polinukleotidaza ili nukleodepolimeraza.[1] Nukleaze se obično dele u endonukleaze i egzonukleaze, mada se neki enzimi mogu svrstati u obe kategorije.
Tokom kasnih 1960-tih, naučnici Stjuart Lin i Verner Arber su izolovali primere dva tipa enzima odgovornih za restrikciju rasta faga kod Escherichia coli bakterija.[2][3] Jedan od tih enzima je dodavao metil grupu na DNK, generišući metilisanu DNK, dok je drugi presecao nemetilisanu DNK na velikom broju različitih lokacija duž molekula. Prvi tip enzima je nazvan metilaza, a drugi restrikciona nukleaza. Ova enzimatska oruđa su važna manipulaciju DNK molekula.
Reference
uredi- ↑ Avery, O.T., MacLeod, C.M., McCarty, M. (1944). Studies on the chemical nature of the substance inducing transformation of pneumococcal types: Induction of transformation by a desoxyribonucleic acid fraction isolated from Pneumococcus type III. J. Exp. Med. 79: 137-158.
- ↑ Linn S., Arber, W. (1968). Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form. Proc. Natl. Acad. Sci. USA. 59:1300-1306
- ↑ Arber, W., Linn S. (1969) DNA modification and restriction. Annu. Rev. Biochem. 38:467-500
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.
