Metabolom

Metabolom^[1] se odnosi na kompletni set malih-molekulskih metabolita (kao što su intermedijeri, hormoni i drugi signalni molekuli, kao i sekundarni metaboliti) koji se može naći u biološkom uzorku, kao što je jednostavni organizam. Reč je skovana po analogiji sa transkriptomikom i proteomikom, kao što su transkriptom i proteom, metabolom je dinamičan, i menja se od sekunde do sekunde. Mada se metabolom može dovoljno jasno definisati, još uvek nije moguće analizirati celokupan opseg metabolita jednim analitičkim metodom. Januara 2007 naučnici sa Alberta univerziteta i Kalgari univerziteta su završili nacrt ljudskog metaboloma. Oni su kategorizovali i karakterizovali 2,500 metabolita, 1,200 lekova i 3,500 komponenti hrane koji se mogu naći u ljudskom telu.^[2]^[3]^[4]

Vidi još uredi

Metabolom tumora
Masena spektrometrija | 2D gel | Sekvenciranje proteina.
Baze podataka: PIR | Swissprot | Pfam
Bioinformatika

Reference uredi

↑ Prva upotreba termina metabolom u literaturi – Oliver SG, Winson MK, Kell DB, Baganz F (September 1998). „Systematic functional analysis of the yeast genome”. Trends Biotechnol. 16 (9): 373–8. DOI:10.1016/S0167-7799(98)01214-1. PMID 9744112.
↑ Prva knjiga o metabolomici – Harrigan, G. G. & Goodacre, R. (eds) (2003). RMetabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis. Kluwer Academic Publishers (Boston). ISBN xxx-xxx.
↑ Fiehn O, Kloska S, Altmann T (February 2001). „Integrated studies on plant biology using multiparallel techniques”. Curr. Opin. Biotechnol. 12 (1): 82–6. DOI:10.1016/S0958-1669(00)00165-8. PMID 11167078.
↑ Fiehn O (2001). „Combining genomics, metabolome analysis, and biochemical modelling to understand metabolic networks”. Comp. Funct. Genomics 2 (3): 155–68. DOI:10.1002/cfg.82. PMC 2447208. PMID 18628911.

Literatura uredi

Harrigan, G. G. & Goodacre, R. (eds) (2003). RMetabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis. Kluwer Academic Publishers (Boston). ISBN xxx-xxx.
Weckwerth W (2003). „Metabolomics in systems biology”. Annu Rev Plant Biol 54: 669–89. DOI:10.1146/annurev.arplant.54.031902.135014. PMID 14503007.
Goodacre R, Vaidyanathan S, Dunn WB, Harrigan GG, Kell DB (May 2004). „Metabolomics by numbers: acquiring and understanding global metabolite data”. Trends Biotechnol. 22 (5): 245–52. DOI:10.1016/j.tibtech.2004.03.007. PMID 15109811.
Wishart DS, Tzur D, Knox C, Eisner R, Guo AC, Young N, Cheng D, Jewell K, Arndt D, Sawhney S, Fung C, Nikolai L, Lewis M, Coutouly M-A, Forsythe I, Tang P, Shrivastava S, Jeroncic K, Stothard P, Amegbey G, Block D, Hau DD, Wagner J, Miniaci J, Clements M, Gebremedhin M, Guo N, Zhang Y, Duggan GE, MacInnis GD, Weljie AM, Dowlatabadi R, Bamforth F, Clive D, Greiner R, Li L, Marrie T, Sykes BD, Vogel HJ, Querengesser L (January 2007). „HMDB: The Human Metabolome Database”. Nucleic Acids Research 35 (Database issue): D521–6. DOI:10.1093/nar/gkl923. PMID 17202168.
Wishart DS, Knox C, Guo AC, Eisner R, Young N, Gautam B, Hau DD, Psychogios N, Dong E, Bouatra S, Mandal R, Sinelnikov I, Xia J, Jia L, Cruz JA, Lim E, Sobsey CA, Shrivastava S, Huang P, Liu P, Fang L, Peng J, Fradette R, Cheng D, Tzur D, Clements M, Lewis A, De Souza A, Zuniga A, Dawe M, Xiong Y, Clive D, Greiner R, Nazyrova A, Shaykhutdinov R, Li L, Vogel HJ, Forsythe I (January 2009). „HMDB: a knowledgebase for the human metabolome”. Nucleic Acids Research 37 (Database issue): D603–10. DOI:10.1093/nar/gkn810. PMID 18953024.
Nicholson JK, Holmes E, Lindon JC, Wilson ID (October 2004). „The challenges of modeling mammalian biocomplexity”. Nat. Biotechnol. 22 (10): 1268–74. DOI:10.1038/nbt1015. PMID 15470467. . Stresses the role of intestinal microorganisms in contributing to the human metabolome.
van der Greef J, Stroobant P, van der Heijden R (October 2004). „The role of analytical sciences in medical systems biology”. Curr Opin Chem Biol 8 (5): 559–65. DOI:10.1016/j.cbpa.2004.08.013. PMID 15450501.
- Važnost analitičkih nauka za omike (engl. omics) i sistemsku biologiju
Kell DB (June 2004). „Metabolomics and systems biology: making sense of the soup”. Curr. Opin. Microbiol. 7 (3): 296–307. DOI:10.1016/j.mib.2004.04.012. PMID 15196499.
Dunn, W.B. and Ellis, D.I. (2005) Metabolomics: current analytical platforms and methodologies. Trends in Analytical Chemistry 24(4), 285-294.
Ellis DI, Goodacre R (August 2006). „Metabolic fingerprinting in disease diagnosis: biomedical applications of infrared and Raman spectroscopy”. Analyst 131 (8): 875–85. DOI:10.1039/b602376m. PMID 17028718.

Spoljašnje veze uredi

Portal Informatika

Proteome.org
Bioinformatika žurnal Arhivirano 2008-11-22 na Wayback Machine-u
Projekat: Ljudski metabolom
baza podataka: Ljudski metabolom
Biblioteka: Ljudski metaboliti
Projekat: Ljudski serum metabolom (HUSERMET) Arhivirano 2014-05-17 na Wayback Machine-u
Standard za objavljivanje metabolomskih studija
Naučnici završili ljudski metabolom Arhivirano 2007-09-30 na Wayback Machine-u

[FirstUse-1] Prva upotreba termina metabolom u literaturi – Oliver SG, Winson MK, Kell DB, Baganz F (September 1998). „Systematic functional analysis of the yeast genome”. Trends Biotechnol. 16 (9): 373–8. DOI:10.1016/S0167-7799(98)01214-1. PMID 9744112.

[2] Prva knjiga o metabolomici – Harrigan, G. G. & Goodacre, R. (eds) (2003). RMetabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis. Kluwer Academic Publishers (Boston). ISBN xxx-xxx.

[3] Fiehn O, Kloska S, Altmann T (February 2001). „Integrated studies on plant biology using multiparallel techniques”. Curr. Opin. Biotechnol. 12 (1): 82–6. DOI:10.1016/S0958-1669(00)00165-8. PMID 11167078.

[4] Fiehn O (2001). „Combining genomics, metabolome analysis, and biochemical modelling to understand metabolic networks”. Comp. Funct. Genomics 2 (3): 155–68. DOI:10.1002/cfg.82. PMC 2447208. PMID 18628911.

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