Glicerol-1-fosfat dehidrogenaza (NAD(P)+)
Glicerol-1-fosfat dehidrogenaza (NAD(P)+) (EC 1.1.1.261, sn-glicerol-1-fosfat:NAD+ oksidoreduktaza, G-1-P dehidrogenaza, Gro1PDH, AraM) je enzim sa sistematskim imenom sn-glicerol-1-fosfat:NAD(P)+ 2-oksidoreduktaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Glicerol-1-fosfat dehidrogenaza (NAD(P)+) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.261 | ||||||||
| CAS broj | 204594-18-3 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- sn-glicerol-1-fosfat + NAD(P)+ gliceron fosfat + NAD(P)H + H+
Ovaj enzim se prvenstveno nalazi u Zn2+-zavisnoj formi kod arheja, dok je Ni2+-zavisna forma prisutna kod Gram-pozitivnih bakterija. Zn2+-zavisni metaloenzim je odgovoran za formiranje arhejski-specifičnog sn-glicerol-1-fosfata, u prvom koraku biosinteze polarnih lipida. On je enantiomer sn-glicerol 3-fosfata, forme glicerofosfata prisutnog kod bakterija i eukariota. Drugi enzimi koji učestvuju u biosintezi polarnih lipida kod arheja su EC 2.5.1.41, fosfoglicerol geranilgeraniltransferaza, i EC 2.5.1.42, (geranilgeranilglicerol-fosfat geranilgeraniltransferaza, koji zajedno alkiliraju hidroksi grupe glicerol 1-fosfata i proizvode nezasićenu arhetidinsku kiselinu, na koju deluje EC 2.7.7.67, CDP-arheolna sintaza, da formira CDP-nezasićeni arheol. Krajnji korak ovog sintetičkog puta je adicija L-serina, sa pratećim uklanjanjem CMP-a, što dovodi do produkcije nezasićenog arhetidilserina. Aktivnost ovog enzima stimuliše K+.
Reference uredi
- ↑ Nishihara, M. and Koga, Y. (1995). „sn-Glycerol-1-phosphate dehydrogenase in Methanobacterium thermoautotrophicum: key enzyme in biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of archaebacteria”. J. Biochem. 117: 933-935. PMID 8586635.
- ↑ Nishihara, M. and Koga, Y. (1997). „Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea”. J. Biochem. 122: 572-576. PMID 9348086.
- ↑ Koga, Y., Kyuragi, T., Nishihara, M. and Sone, N. (1998). „Did archaeal and bacterial cells arise independently from noncellular precursors? A hypothesis stating that the advent of membrane phospholipid with enantiomeric glycerophosphate backbones caused the separation of the two lines of descent”. J. Mol. Evol. 46: 54-63. PMID 9419225.
- ↑ Morii, H., Nishihara, M. and Koga, Y. (2000). „CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase in the methanogenic archaeon Methanothermobacter thermoautotrophicus”. J. Biol. Chem. 275: 36568-36574. PMID 10960477.
- ↑ Han, J.S. and Ishikawa, K. (2005). „Active site of Zn2+-dependent sn-glycerol-1-phosphate dehydrogenase from Aeropyrum pernix K1”. Archaea 1: 311-317. PMID 15876564.
- ↑ Guldan, H., Sterner, R. and Babinger, P. (2008). „Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis”. Biochemistry 47: 7376-7384. PMID 18558723.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
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