Glicerol-1-fosfat dehidrogenaza (NAD(P)+)

Glicerol-1-fosfat dehidrogenaza (NAD(P)+)
Identifikatori
EC broj	1.1.1.261
CAS broj	204594-18-3
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga
PMC	articles
PubMed	articles
NCBI Protein	search

Glicerol-1-fosfat dehidrogenaza (NAD(P)⁺) (EC 1.1.1.261, sn-glicerol-1-fosfat:NAD⁺ oksidoreduktaza, G-1-P dehidrogenaza, Gro1PDH, AraM) je enzim sa sistematskim imenom sn-glicerol-1-fosfat:NAD(P)⁺ 2-oksidoreduktaza.^[1]^[2]^[3]^[4]^[5]^[6] Ovaj enzim katalizuje sledeću hemijsku reakciju

sn-glicerol-1-fosfat + NAD(P)+

\rightleftharpoons

gliceron fosfat + NAD(P)H + H⁺

Ovaj enzim se prvenstveno nalazi u Zn²⁺-zavisnoj formi kod arheja, dok je Ni²⁺-zavisna forma prisutna kod Gram-pozitivnih bakterija. Zn²⁺-zavisni metaloenzim je odgovoran za formiranje arhejski-specifičnog sn-glicerol-1-fosfata, u prvom koraku biosinteze polarnih lipida. On je enantiomer sn-glicerol 3-fosfata, forme glicerofosfata prisutnog kod bakterija i eukariota. Drugi enzimi koji učestvuju u biosintezi polarnih lipida kod arheja su EC 2.5.1.41, fosfoglicerol geranilgeraniltransferaza, i EC 2.5.1.42, (geranilgeranilglicerol-fosfat geranilgeraniltransferaza, koji zajedno alkiliraju hidroksi grupe glicerol 1-fosfata i proizvode nezasićenu arhetidinsku kiselinu, na koju deluje EC 2.7.7.67, CDP-arheolna sintaza, da formira CDP-nezasićeni arheol. Krajnji korak ovog sintetičkog puta je adicija L-serina, sa pratećim uklanjanjem CMP-a, što dovodi do produkcije nezasićenog arhetidilserina. Aktivnost ovog enzima stimuliše K⁺.

Reference

↑ Nishihara, M. and Koga, Y. (1995). „sn-Glycerol-1-phosphate dehydrogenase in Methanobacterium thermoautotrophicum: key enzyme in biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of archaebacteria”. J. Biochem. 117: 933-935. PMID 8586635.
↑ Nishihara, M. and Koga, Y. (1997). „Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea”. J. Biochem. 122: 572-576. PMID 9348086.
↑ Koga, Y., Kyuragi, T., Nishihara, M. and Sone, N. (1998). „Did archaeal and bacterial cells arise independently from noncellular precursors? A hypothesis stating that the advent of membrane phospholipid with enantiomeric glycerophosphate backbones caused the separation of the two lines of descent”. J. Mol. Evol. 46: 54-63. PMID 9419225.
↑ Morii, H., Nishihara, M. and Koga, Y. (2000). „CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase in the methanogenic archaeon Methanothermobacter thermoautotrophicus”. J. Biol. Chem. 275: 36568-36574. PMID 10960477.
↑ Han, J.S. and Ishikawa, K. (2005). „Active site of Zn²⁺-dependent sn-glycerol-1-phosphate dehydrogenase from Aeropyrum pernix K1”. Archaea 1: 311-317. PMID 15876564.
↑ Guldan, H., Sterner, R. and Babinger, P. (2008). „Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis”. Biochemistry 47: 7376-7384. PMID 18558723.

Literatura

Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Spoljašnje veze

MeSH glycerol-1-phosphate+dehydrogenase

[1] Nishihara, M. and Koga, Y. (1995). „sn-Glycerol-1-phosphate dehydrogenase in Methanobacterium thermoautotrophicum: key enzyme in biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of archaebacteria”. J. Biochem. 117: 933-935. PMID 8586635.

[2] Nishihara, M. and Koga, Y. (1997). „Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea”. J. Biochem. 122: 572-576. PMID 9348086.

[3] Koga, Y., Kyuragi, T., Nishihara, M. and Sone, N. (1998). „Did archaeal and bacterial cells arise independently from noncellular precursors? A hypothesis stating that the advent of membrane phospholipid with enantiomeric glycerophosphate backbones caused the separation of the two lines of descent”. J. Mol. Evol. 46: 54-63. PMID 9419225.

[4] Morii, H., Nishihara, M. and Koga, Y. (2000). „CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase in the methanogenic archaeon Methanothermobacter thermoautotrophicus”. J. Biol. Chem. 275: 36568-36574. PMID 10960477.

[5] Han, J.S. and Ishikawa, K. (2005). „Active site of Zn²⁺-dependent sn-glycerol-1-phosphate dehydrogenase from Aeropyrum pernix K1”. Archaea 1: 311-317. PMID 15876564.

[6] Guldan, H., Sterner, R. and Babinger, P. (2008). „Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis”. Biochemistry 47: 7376-7384. PMID 18558723.

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