Acil-homoserin-lakton sintaza
Acil-homoserin-lakton sintaza (EC 2.3.1.184, acil-homoserin laktonska sintaza, acil homoserin laktonska sintaza, acil-homoserinlakton sintaza, acilhomoserin laktonska sintaza, AHL sintaza, AHS, AHSL sintaza, AhyI, AinS, AinS protein, autoinducer sintaza, autoinducer sinteza protein rhlI, EsaI, ExpISCC1, ExpISCC3065, LasI, LasR, LuxI, LuxI protein, LuxM, N-acil homoserin lakton sintaza, RhlI, YspI, acil-(acil nosilac protein):S-adenozil-L-metionin aciltranseraza (formira lakton, otpušta metiltioadenozin)) je enzim sa sistematskim imenom acil-(acil-nosilac protein):S-adenozil-L-metionin aciltranseraza (formira lakton).[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Acil-homoserin-lakton sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.3.1.184 | ||||||||
| CAS broj | 176023-66-8 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- acil-[acil-nosilac protein] + S-adenozil-L-metionin [acil-nosilac protein] + S-metil-5'-tioadenozin + N-acil-L-homoserin lakton
Acil-homoserinske laktone formiraju brojne bakterijske vrste. Oni se koriste za regulaciju izražavanja gena virulencije u procesu poznatom kao očitavanje kvoruma.
Reference uredi
- ↑ Schaefer, A.L., Val, D.L., Hanzelka, B.L., Cronan, J.E., Jr. and Greenberg, E.P. (1996). „Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein”. Proc. Natl. Acad. Sci. USA 93: 9505-9509. PMID 8790360.
- ↑ Watson, W.T., Murphy, F.V., 4th, Gould, T.A., Jambeck, P., Val, D.L., Cronan, J.E., Jr., Beck von Bodman, S. and Churchill, M.E. (2001). „Crystallization and rhenium MAD phasing of the acyl-homoserinelactone synthase EsaI”. Acta Crystallogr. D Biol. Crystallogr. 57: 1945-1949. PMID 11717525.
- ↑ Chakrabarti, S. and Sowdhamini, R. (2003). „Functional sites and evolutionary connections of acylhomoserine lactone synthases”. Protein Eng. 16: 271-278. PMID 12736370.
- ↑ Hanzelka, B.L., Parsek, M.R., Val, D.L., Dunlap, P.V., Cronan, J.E., Jr. and Greenberg, E.P. (1999). „Acylhomoserine lactone synthase activity of the Vibrio fischeri AinS protein”. J. Bacteriol. 181: 5766-5770. PMID 10482519.
- ↑ Parsek, M.R., Val, D.L., Hanzelka, B.L., Cronan, J.E., Jr. and Greenberg, E.P. (1999). „Acyl homoserine-lactone quorum-sensing signal generation”. Proc. Natl. Acad. Sci. USA 96: 4360-4365. PMID 10200267.
- ↑ Ulrich, R.L. (2004). „Quorum quenching: enzymatic disruption of N-acylhomoserine lactone-mediated bacterial communication in Burkholderia thailandensis”. Appl. Environ. Microbiol. 70: 6173-6180. PMID 15466564.
- ↑ Gould, T.A., Schweizer, H.P. and Churchill, M.E. (2004). „Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI”. Mol. Microbiol. 53: 1135-1146. PMID 15306017.
- ↑ Raychaudhuri, A., Jerga, A. and Tipton, P.A. (2005). „Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa”. Biochemistry 44: 2974-2981. PMID 15723540.
- ↑ Gould, T.A., Herman, J., Krank, J., Murphy, R.C. and Churchill, M.E. (2006). „Specificity of acyl-homoserine lactone synthases examined by mass spectrometry”. J. Bacteriol. 188: 773-783. PMID 16385066.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.