23S rRNK (guanin748-N1)-metiltransferaza
23S rRNK (guanin748-N1)-metiltransferaza (EC 2.1.1.188, Rlma(II), Rlma2, 23S rRNK m1G748 metiltransferaza, RlmaII, Rlma II, metiltransferaza tilozinske otpornosti RlmA(II), TlrB, metiltransferaza II velike podjedinice rRNK) je enzim sa sistematskim imenom S-adenozil-L-metionin:23S rRNK (guanin748-N1)-metiltransferaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 23S rRNK (guanin748-N1)-metiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.188 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- S-adenozil-L-metionin + guanin748 u 23S rRNK S-adenozil-L-homocistein + N1-metilguanin748 u 23S rRNK
Ovaj enzim specifično metiliše guanin748 u N1 poziciji 23S rRNK. Metiltransferaza RlmAII pruža otpornost na makrolidni antibiotik tilozin u otpornoj vrsti bakterije Streptomyces fradiae.
Reference uredi
- ↑ Douthwaite, S., Crain, P.F., Liu, M. and Poehlsgaard, J. (2004). „The tylosin-resistance methyltransferase RlmAII (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748”. J. Mol. Biol. 337: 1073-1077. PMID 15046978.
- ↑ Liu, M., Kirpekar, F., Van Wezel, G.P. and Douthwaite, S. (2000). „The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA”. Mol. Microbiol. 37: 811-820. PMID 10972803.
- ↑ Lebars, I., Yoshizawa, S., Stenholm, A.R., Guittet, E., Douthwaite, S. and Fourmy, D. (2003). „Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII”. EMBO J. 22: 183-192. PMID 12514124.
- ↑ Lebars, I., Husson, C., Yoshizawa, S., Douthwaite, S. and Fourmy, D. (2007). „Recognition elements in rRNA for the tylosin resistance methyltransferase RlmAII”. J. Mol. Biol. 372: 525-534. PMID 17673230.
- ↑ Douthwaite, S., Jakobsen, L., Yoshizawa, S. and Fourmy, D. (2008). „Interaction of the tylosin-resistance methyltransferase RlmAII at its rRNA target differs from the orthologue RlmAI”. J. Mol. Biol. 378: 969-975. PMID 18406425.
- ↑ Liu, M. and Douthwaite, S. (2002). „Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria”. Mol. Microbiol. 44: 195-204. PMID 11967079.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.