23S rRNK (citidin1920-2'-O)-metiltransferaza
23S rRNK (citidin1920-2'-O)-metiltransferaza (EC 2.1.1.226, TlyA (nespecifična)) je enzim sa sistematskim imenom S-adenozil-L-metionin:23S rRNK (citidin1920-2'-O)-metiltransferaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 23S rRNK (citidin1920-2'-O)-metiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.226 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- S-adenozil-L-metionin + citidin1920 u 23S rRNK S-adenozil-L-homocistein + 2'-O-metilcitidin1920 u 23S rRNK
Bifunkcionalni enzim iz Mycobakterija tuberculosis 2'-O-metiliše citidin1920 u heliksu 69 u 23S rRNK i citidin1409 u heliksu 44 u 16S rRNK (cf. EC 2.1.1.227, 16S rRNK (citidin1409-2'-O)-metiltransferaza). Ove metilacije rezultiraju u povećanoj podložnosti na antibiotike kapreomicin i viomicin.
Reference uredi
- ↑ Johansen, S.K., Maus, C.E., Plikaytis, B.B. and Douthwaite, S. (2006). „Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2′-O-methylations in 16S and 23S rRNAs”. Mol. Cell 23: 173-182. PMID 16857584.
- ↑ Maus, C.E., Plikaytis, B.B. and Shinnick, T.M. (2005). „Mutation of tlyA confers capreomycin resistance in Mycobacterium tuberculosis”. Antimicrob. Agents Chemother. 49: 571-577. PMID 15673735.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.