Voćni bromelain
Voćni bromelain (EC 3.4.22.33, bromelain voćnog soka, ananaza, bromelaza, bromelin, ekstranaza, pinaza, ananasni enzim, traumanaza, voćni bromelain FA2) je enzim.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Voćni bromelain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.22.33 | ||||||||
| CAS broj | 9001-00-7 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- Hidroliza proteina sa širokom specifičnošću za peptidne veze. Bz-Phe-Val-Arg-NHMec je dobar sintetički supstrat. Enzim ne deluje na Z-Arg-Arg-NHMec (c.f. matični bromelain)
Ovaj enzim je izolovan iz biljke ananasa, Ananas comosus.
Reference uredi
- ↑ Sasaki, M., Kato, T. and Iida, S. (1973). „Antigenic determinant common to four kinds of thiol proteases of plant origin”. J. Biochem. (Tokyo) 74: 635-637. PMID 4127920.
- ↑ Yamada, F., Takahashi, N. and Murachi, T. (1976). „Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2”. J. Biochem. (Tokyo) 79: 1223-1234. PMID 956152.
- ↑ Ota, S., Muta, E., Katanita, Y. and Okamoto, Y. (1985). „Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains”. J. Biochem. (Tokyo) 98: 219-228. PMID 4044551.
- ↑ Rowan, A.D., Buttle, D.J. and Barrett, A.J. (1990). „The cysteine proteinases of the pineapple plant”. Biochem. J. 266: 869-875. PMID 2327970.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.