Sortaza-A
Sortaza-A (EC 3.4.22.70, SrtA, SrtA protein, SrtA sortaza) je enzim.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Sortaza-A | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.22.70 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- Ovaj enzim enzim katalizuje reakciju sortiranja ćelijskog zida u kojoj se površinski protein sa signalom sortiranja koji sadrži LPXTG motif razlaže između Thr i Gly ostataka. Rezultirajući treoninski karboksilni kraj proteina je kovalentno vezan za pentaglicin peptidoglikana.
Ovaj enzim pripada peptidaznoj familiji C60.
Reference uredi
- ↑ Ton-That, H., Liu, G., Mazmanian, S.K., Faull, K.F. and Schneewind, O. (1999). „Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif”. Proc. Natl. Acad. Sci. USA 96: 12424-12429. PMID 10535938.
- ↑ Zong, Y., Bice, T.W., Ton-That, H., Schneewind, O. and Narayana, S.V. (2004). „Crystal structures of Staphylococcus aureus sortase A and its substrate complex”. J. Biol. Chem. 279: 31383-31389. PMID 15117963.
- ↑ Race, P.R., Bentley, M.L., Melvin, J.A., Crow, A., Hughes, R.K., Smith, W.D., Sessions, R.B., Kehoe, M.A., McCafferty, D.G. and Banfield, M.J. (2009). „Crystal structure of Streptococcus pyogenes sortase A: implications for sortase mechanism”. J. Biol. Chem. 284: 6924-6933. PMID 19129180.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.