Pirimidin oksigenaza
Pirimidin oksigenaza (EC 1.14.99.46, RUTA) je enzim sa sistematskim imenom uracil,FMNH2:kiseonik oksidoreduktaza (uracilna hidroksilacija, otvaranje prstena).[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Pirimidin oksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.99.46 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) uracil + FMNH2 + O2 (Z)-3-ureidoakrilatna perkiselina + FMN
- (2) timin + FMNH2 + O2 (Z)-2-metilureidoakrilatna perkiselina + FMN
In vitro se produkt (Z)-3-ureidoakrilat perkiselina spontano redukuje do ureidoakrilata.
Reference uredi
- ↑ Mukherjee, T., Zhang, Y., Abdelwahed, S., Ealick, S.E. and Begley, T.P. (2010). „Catalysis of a flavoenzyme-mediated amide hydrolysis”. J. Am. Chem. Soc. 132: 5550-5551. PMID 20369853.
- ↑ Kim, K.S., Pelton, J.G., Inwood, W.B., Andersen, U., Kustu, S. and Wemmer, D.E. (2010). „The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems”. J. Bacteriol. 192: 4089-4102. PMID 20400551.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.