Nisko-specifična L-treoninska aldolaza
Nisko-specifična L-treoninska aldolaza (EC 4.1.2.48, LtaE) je enzim sa sistematskim imenom L-treonin/L-allo-treonin acetaldehid-lijaza (formira glicin).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Nisko-specifična L-treoninska aldolaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.1.2.48 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) L-treonin glicin + acetaldehid
- (2) L-allo-treonin glicin + acetaldehid
Za dejstvo ovog enzima je neophodan piridoksal fosfat.
Reference uredi
- ↑ Yamada, H., Kumagai, H., Nagate, T. and Yoshida, H. (1970). „Crystalline threonine aldolase from Candida humicola”. Biochem. Biophys. Res. Commun. 39: 53-58. PMID 5438301.
- ↑ Kumagai, H., Nagate, T., Yoshida, H. and Yamada, H. (1972). „Threonine aldolase from Candida humicola. II. Purification, crystallization and properties”. Biochim. Biophys. Acta 258: 779-790. PMID 5017702.
- ↑ Liu, J.Q., Nagata, S., Dairi, T., Misono, H., Shimizu, S. and Yamada, H. (1997). „The GLY1 gene of Saccharomyces cerevisiae encodes a low-specific L-threonine aldolase that catalyzes cleavage of L-allo-threonine and L-threonine to glycine–expression of the gene in Escherichia coli and purification and characterization of the enzyme”. Eur. J. Biochem. 245: 289-293. PMID 9151955.
- ↑ Liu, J.Q., Dairi, T., Itoh, N., Kataoka, M., Shimizu, S. and Yamada, H. (1998). „Gene cloning, biochemical characterization and physiological role of a thermostable low-specificity L-threonine aldolase from Escherichia coli”. Eur. J. Biochem. 255: 220-226. PMID 9692922.
- ↑ Kim, J., Kershner, J.P., Novikov, Y., Shoemaker, R.K. and Copley, S.D. (2010). „Three serendipitous pathways in E. coli can bypass a block in pyridoxal-5′-phosphate synthesis”. Mol. Syst. Biol. 6: #436-436. PMID 21119630.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.