Holesterol monooksigenaza
Holesterol monooksigenaza (EC 1.14.15.6, holesterolna dezmolaza, citohrom P-450scc, dezmolaza, steroid 20-22, enzim odvajanja C27-bočnog lanca, holesterolna 20-22-dezmolaza, holesterolna C20-22 dezmolaza, enzim odvajanja holesterolnog bočnog lanca, enzim odvajanja holesterolnog bočnog lanca, steroidna 20-22 dezmolaza, steroidna 20-22-lijaza) je enzim sa sistematskim imenom holesterol,redukovani-adrenal-feredoksin:kiseonik oksidoreduktaza (odvajanje bočnog lanca).[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Holesterol monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.15.6 | ||||||||
| CAS broj | 440354-98-3&title= 37292-81-2, 440354-98-3 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- holesterol + redukovani adrenal feredoksin + O2 pregnenolon + 4-metilpentanal + oksidovani adrenal feredoksin + H2O
Ovaj enzim je hem-tiolatni protein. Reakcija se odvija u tri stupnja, hidroksilacija u C-20 i C-22 prethodi presecanu bočnog lancu u poziciji C-20.
Reference uredi
- ↑ Hanukoglu, I. and Jefcoate, C.R. (1980). „Mitochondrial cytochrome P-450sec. Mechanism of electron transport by adrenodoxin”. J. Biol. Chem. 255: 3057-3061. PMID 6766943.
- ↑ Hanukoglu, I., Spitsberg, V., Bumpus, J.A., Dus, K.M. and Jefcoate, C.R. (1981). „Adrenal mitochondrial cytochrome P-450scc. Cholesterol and adrenodoxin interactions at equilibrium and during turnover”. J. Biol. Chem. 256: 4321-4328. PMID 7217084.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.