Glutation amid reduktaza
Glutation amid reduktaza (EC 1.8.1.16, GAR) je enzim sa sistematskim imenom glutation amid:NAD+ oksidoreduktaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Glutation amid reduktaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.8.1.16 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 2 glutation amid + NAD+ glutation amid disulfid + NADH + H+
Ovaj enzim je dimerni flavoprotein (FAD). On obnavlja glutation amid disulfid, koji se formira tokom redukcije peroksida enzimom EC 1.11.1.17 (glutation amid-zavisnom peroksidazom), nazad u glutationski amid (on katalizuje reakciju reverznom smeru od prikazanog).
Reference uredi
- ↑ Vergauwen, B., Pauwels, F., Jacquemotte, F., Meyer, T.E., Cusanovich, M.A., Bartsch, R.G. and Van Beeumen, J.J. (2001). „Characterization of glutathione amide reductase from Chromatium gracile. Identification of a novel thiol peroxidase (Prx/Grx) fueled by glutathione amide redox cycling”. J. Biol. Chem. 276: 20890-20897. PMID 11399772.
- ↑ Vergauwen, B., Van Petegem, F., Remaut, H., Pauwels, F. and Van Beeumen, J.J. (2002). „Crystallization and preliminary X-ray crystallographic analysis of glutathione amide reductase from Chromatium gracile”. Acta Crystallogr. D Biol. Crystallogr. 58: 339-340. PMID 11807270.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.