Fosfatidilholin—retinol O-aciltransferaza
Fosfatidilholin—retinol O-aciltransferaza (EC 2.3.1.135, lecitin---retinol aciltransferaza, fosfatidilholin:retinol-(ćelijski-retinol-vezujući-protein) O-aciltransferaza, lecitin:retinol aciltransferaza, lecitin-retinol aciltransferaza, retinil estarska sintaza, LRAT, lecitin retinol acilna transferaza) je enzim sa sistematskim imenom fosfatidilholin:retinol—(cellular-retinol-binding-protein) O-aciltransferaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Fosfatidilholin—retinol O-aciltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.3.1.135 | ||||||||
| CAS broj | 117444-03-8 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- fosfatidilholin + retinol---[ćelijski-retinol-vezujući-protein] 2-acilglicerofosfoholin + retinil-estar---[ćelijski-retinol-vezujući-protein]
Ovaj enzim je ključan za retinoidni metabolism. On katalizuje transfer acil grupa sa sn-1 pozicija fosfatidilholina na retinol, čime se formiraju retinil estari, koji se zatim skladište. On prepoznaje supstrat u slobodnoj formi i vezan za ćelijski-retinol-vezujući-protein, ali ima veći afinitet za vezanu formu. Ovaj ezim takođe esterifikuje 11-cis-retinol.
Reference uredi
- ↑ MacDonald, P.N. and Ong, D.E. (1988). „Evidence for a lecithin-retinol acyltransferase activity in the rat small intestine”. J. Biol. Chem. 263: 12478-12482. PMID 3410848.
- ↑ Saari, J.C. and Bredberg, D.L. (1989). „Lecithin:retinol acyltransferase in retinal pigment epithelial microsomes”. J. Biol. Chem. 264: 8636-8340. PMID 2722792.
- ↑ Saari, J.C., Bredberg, D.L. and Farrell, D.F. (1993). „Retinol esterification in bovine retinal pigment epithelium: reversibility of lecithin:retinol acyltransferase”. Biochem. J. 291: 697-700. PMID 8489497.
- ↑ Mata, N.L. and Tsin, A.T. (1998). „Distribution of 11-cis LRAT, 11-cis RD and 11-cis REH in bovine retinal pigment epithelium membranes”. Biochim. Biophys. Acta 1394: 16-22. PMID 9767084.
- ↑ Ruiz, A., Winston, A., Lim, Y.H., Gilbert, B.A., Rando, R.R. and Bok, D. (1999). „Molecular and biochemical characterization of lecithin retinol acyltransferase”. J. Biol. Chem. 274: 3834-3841. PMID 9920938.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.