Flavonol sintaza
Flavonol sintaza (EC 1.14.11.23, flavonolna sintaza) je enzim sa sistematskim imenom dihidroflavonol,2-oksoglutarat:kiseonik oksidoreduktaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju:
| Flavonol sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.11.23 | ||||||||
| CAS broj | 146359-76-4 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Osim desaturacije (2R,3R)-dihidroflavonola do flavonola, enzim iz ploda Citrus unshiu takođe ispoljava nespecifičnu aktivnost koja trans-hidroksiluje flavanon (2S)-naringenin i veštački (2R)-naringenin u C-3 do kampferol i (2R,3R)-dihidrokampferol. Za rad ovog enzima je neophodan Fe2+ jon.
Reference uredi
- ↑ Wellmann, F. (2002). „, Lukačin, R., Moriguchi, T., Britsch, L., Schiltz, E. and Matern, U. Functional expression and mutational analysis of flavonol synthase from Citrus unshiu”. Eur. J. Biochem. 269: 4134-4142. PMID 12180990.
- ↑ Lukačin, R., Wellmann, F., Britsch, L., Martens, S. and Matern, U. (2003). „Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase”. Phytochemistry 62: 287-292. PMID 12620339.
- ↑ Martens, S., Forkmann, G., Britsch, L., Wellmann, F., Matern, U. and Lukačin, R. (2003). „Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley”. FEBS Lett. 544: 93-98. PMID 12782296.
- ↑ Turnbull, J.J., Nakajima, J., Welford, R.W., Yamazaki, M., Saito, K. and Schofield, C.J. (2004). „Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3β-hydroxylase”. J. Biol. Chem. 279: 1206-1216. PMID 14570878.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.