Beta-porfiranaza
Beta-porfiranaza (EC 3.2.1.178, porfiranaza, PorA, PorB, endo-beta-porfiranaza) je enzim sa sistematskim imenom porfiran beta-D-galaktopiranoza-(1->4)-alfa-L-galaktopiranoza-6-sulfat 4-glikanohidrolaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Beta-porfiranaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.2.1.178 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- hidroliza beta-D-galaktopiranoza-(1->4)-alfa-L-galaktopiranoza-6-sulfatnih veza u porfiranu
Osnova porfirana se uglavnom (~70%) sastoji od (1->3)-vezane beta-D-galaktopiranoze kojoj sledi (1->4)-vezana alfa-L-galaktopiranoza-6-sulfat [drugih 30% su uglavnom agarobiozne ponavljajuće jedinice (1->3)-vezane beta-D-galaktopiranoze kojoj sledi (1->4)-vezana 3,6-anhidro-alfa-L-galaktopiranoza]. Ovaj enzim razlaže (1->4) veze između beta-D-galaktopiranoze i alfa-L-galaktopiranoza-6-sulfata, i uglavnom formira disaharid alfa-L-galaktopiranoza-6-sulfat-(1->3)-beta-D-galaktozu, mada neki duži oligosaharidi sa parnim brojem ostataka su takođe mogući.
Reference uredi
- ↑ Hehemann, J.H., Correc, G., Barbeyron, T., Helbert, W., Czjzek, M. and Michel, G. (2010). „Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota”. Nature 464: 908-912. PMID 20376150.
- ↑ Correc, G., Hehemann, J.H., Czjzek, M. and Helbert, W. (2011). „Structural analysis of the degradation products of porphyran digested by Zobellia galactanivorans β-porphyranase A”. Carbohydrate Polymers 83: 277-283.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.