Alifatična (R)-hidroksinitrilna lijaza
Alifatična (R)-hidroksinitrilna lijaza (EC 4.1.2.46, (R)-HNL, (R)-oksinitrilaza, (R)-hidroksinitril lijaza, LuHNL) je enzim sa sistematskim imenom (2R)-2-hidroksi-2-metilbutannitril butan-2-on-lijaza (formira cijanid).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Alifatična (R)-hidroksinitrilna lijaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.1.2.46 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (2R)-2-hidroksi-2-metilbutannitril cijanid + butan-2-on
Ovaj enzim sadrži Zn2+.
Reference uredi
- ↑ Trummler, K., Roos, J., Schwaneberg, U., Effenberger, F., Förster, S., Pfizenmaier, K. and Wajant, H. (1998). „Expression of the Zn2+-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris– utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis”. Plant Sci. 139: 19-27.
- ↑ Trummler, K. and Wajant, H. (1997). „Molecular cloning of acetone cyanohydrin lyase from flax (Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases”. J. Biol. Chem. 272: 4770-4774. PMID 9030531.
- ↑ Albrecht, J., Jansen, I.and Kula, M.R. (1993). „Improved purification of an (R)-oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range”. Biotechnol. Appl. Biochem. 17: 191-203.
- ↑ Xu, L.-L., Singh, B.K. and Conn, E.E. (1988). „Purification and characterization of acetone cyanohydrin lyase from Linum usitatissimum”. Arch. Biochem. Biophys. 263: 256-263. PMID 3377504.
- ↑ Cabirol, F.L., Tan, P.L., Tay, B., Cheng, S., Hanefeld, U. and Sheldon, R.A. (2008). „Linum usitatissimum hydroxynitrile lyase cross-linked enzyme aggregates: a recyclable enantioselective catalyst”. Adv. Synth. Catal. 350: 2329-2338.
- ↑ Breithaupt, H., Pohl, M., Bönigk, W., Heim, P., Schimz, K.-L. and Kula, M.-R. (1999). „Cloning and expression of (R)-hydroxynitrile lyase from Linum usitatissimum (flax)”. J. Mol. Catal. B 6: 315-332.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.