2-oksoglutarat dioksigenaza (formira etilen)
2-oksoglutarat dioksigenaza (formira etilen) (EC 1.13.12.19, etilen-formirajući enzim, EFE) je enzim sa sistematskim imenom 2-oksoglutarat:kiseonik oksidoreduktaza (formira dekarboksilacija, etilen).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 2-oksoglutarat dioksigenaza (formira etilen) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.13.12.19 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 2-oksoglutarat + O2 etilen + 3 CO2 +H2O
Ova je jedna od dve simultane reakcije koje katalizuje ovaj enzim, koji je odgovoran za formiranje etilena kod bakterija iz grupe Pseudomonas syringae. U drugoj reakciji (EC 1.14.11.34, 2-oksoglutarat/L-arginin monooksigenaza/dekarboksilaza) ovaj enzim katalizuje monooksigenaciju 2-oksoglutarata i L-arginina, formirajući sukcinat, ugljen dioksid i L-hidroksiarginin, koji se naknadno razlaže u guanidin i (S)-1-pirolin-5-karboksilat.
Reference uredi
- ↑ Nagahama, K., Ogawa, T., Fujii, T., Tazaki, M., Tanase, S., Morino, Y. and Fukuda, H. (1991). „Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2”. J. Gen. Microbiol. 137: 2281-2286. PMID 1770346.
- ↑ Fukuda, H., Ogawa, T., Tazaki, M., Nagahama, K., Fujii, T., Tanase, S. and Morino, Y. (1992). „Two reactions are simultaneously catalyzed by a single enzyme: the arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae”. Biochem. Biophys. Res. Commun. 188: 483-489. PMID 1445291.
- ↑ Fukuda, H., Ogawa, T., Ishihara, K., Fujii, T., Nagahama, K., Omata, T., Inoue, Y., Tanase, S. and Morino, Y. (1992). „Molecular cloning in Escherichia coli, expression, and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2”. Biochem. Biophys. Res. Commun. 188: 826-832. PMID 1445325.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.